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![]() ![]() ![]() Videotape Editors: Evamarie Keller, Linda M.Graphics Animation: Michael Fleming, Linda Hamil Here are the previews of Barneys Campfire Sing-Along. Barney Classic Collection VHS Tape Campfire Sing Along (11) 9.99 Free shipping Barneys Campfire Sing-Along VHS 1990 Video Tape Kids Tested 12.99 3.Swing Crew: Santiago Luna, Abdon Molina.Content Consultants: Valeria Lovelace, Ph.D., Bettina Peel.Production Assistants: Genia Christine, Cher Jung, Tim Carter, Paul Lacy, Ruth Luwisch, Walter Jacob.Assistants to the Producers: Danette DeSena, Carol D.Sound Effects: Dick Maitland, Roy Carch Get the best deals for barney campfire sing-along 1990 vhs at.Camera Operators: Jim Conrad, Patrick Gutierrez.Scenic Design and Specialty Props: Max Cooksey. ![]()
![]() ![]() Once the crew lock was completely depressurized, Fincke and Chamitoff took their EMUs to battery power and officially began the final Space Shuttle crew spacewalk in history at 4:02 Central time. ![]() Once the crew lock of Quest was depressurized to 10.2 psi, spacesuit purge was conducted as the spacewalking duo donned their tell-tale white EMUs (Extravehicular Mobility Units). See AlsoĪfter a brief, 30-minute hygiene break, Fincke and Chamitoff returned to Quest to begin final preparations for their spacewalk, or EVA – Extra-Vehicular Activity. With Flight Day 12 (FD-12) for Endeavour and her STS-134 crew come four important and truly historic milestones: the final EVA/Spacewalk of the Space Shuttle Program, the final Station construction EVA by non-ISS crewmembers (as currently scheduled), the permanent transfer of Endeavour’s Orbiter Boom Sensor System from the Shuttle itself to the International Space Station, and the 1000th hour of spacewalking time dedicated to construction of the International Space Station.Īfter waking up at 1956 EDT, Mike Fincke and Greg Chamitoff emerged from the Quest Airlock where they spent the night “camping out” for their final foray into the vacuum of space. This historic day concluded all scheduled EVA activities for the Space Shuttle Program and its astronauts since EVAs on Shuttle missions began on the STS-6 (1983) mission by Shuttle orbiter Challenger. As Endeavour heads into the sunset portion of her historic final voyage to space, her crew have completed one of the biggest lasts in the Shuttle Program: the 162nd and FINAL Space Shuttle spacewalk. ![]() ![]() Now, when we preview, everything plays the way we want it to. Select the second animation which brings in the gravity text, and choose Fade. Select the first animation, and in Effect Options, select From Top. So now we know the first animation brings in the gravity arrow. ![]() Select Animation 1, which brings in the bar, and delete it.Ĭlick Play from. Then, in Effect Options, choose One by One. In this one, it would make sense to animate the gravity arrow flying down and the lift arrow flying up, and the text fading in. Then, choose the sequence in which you want to bring the elements in.įor example, Level at Once brings all the elements in each level on one at a time. ![]() We could use a Fade or Wipe animation for this organization chart, but something like a Zoom would work too. You choose an animation that makes sense for how you want to bring in or emphasize the elements of the graphic. What kind of animation do you think would work with a scatter chart? You can animate a pie chart, using the same logic, except you will use a Wheel animation, which wipes on the chart like a clock. Now when we preview the animation, the grid is static. Select the first animation, which is the one for the grid. These numbers correspond to the four animations associated with the chart. And what we want is for the grid to be static. The only thing wrong is that the grid animates on too. So, when we talk about the differences between the years, we can bring the three series in one at a time. Let's stick with the upward direction, so it appears the columns grow, as they animate on.ĭown here, we can choose to have all the elements in each series, or category animate on at once, or each element in a series or category animate on. In this column chart, animations like Fly In or Bounce don't do much to help an audience visualize the data.īut, let's try a Wipe. ![]() Charts and SmartArt are similar to text as you can animate elements individually.įor charts, the trick is to find an animation that makes sense with the chart style. ![]() ![]() Asides from the plastic which dominates most of the merchandise for The Golden Compass movie, some higher-end replicas of props from the film are being produced. ![]() Thread starter Afr0 Start date Tags alethiometer golden compass his dark materials Afr0 New Member. houses for rent in fort pierce antonio's pizzeria loschiavo top trading nations in the world what is the distance between plants per row En chemin, faites la rencontre des 3 dragons de la Khaleesi sur les grandes tendues du Royaume des Sept Couronnes. It has taken seven craftsmen about a year to make the model and it. "This is about as perfect and as real as an Alethiometer can get short of actually being able to commincate with the divine"The pictures do a much better job at describing this item than I ever could.Noble collection alethiometer best external hard drive for ipad pro usb-c All of the three knobs work as designed.they allow the red face "needles" to be positioned around the dial The push button spins the "truth needle" as designed Lid snaps fully shut and button to open latch functions flawlessly This is just a fantastic replica.so close to the original item that here is one owner's quote. Glass face is clean, clear and undamaged. It has a hinged lid that opens to a 90 degree vertical position exposing the Alethiometer face and dials Three ornate swirled orb control knobs on the edge control the dials that are used by Lyra to set a question to be answered by the compass There is small push button that is part of one of the swirled orb control knobs that when pressed.releases the Alethiometer's "inner truth" needle that spins and finally randomly points to one or more of the pictographs positioned around the edges.this is the Golden Compass's answer (the dark blue "inner truth" needle is reloaded and ready to spin each time the lid is closed and opened)Case is in excellent to near new condition. ![]() This incredible piece has a tarnish proof, lacquered metal case in a gorgeous brushed/antiqued brass finish Superbly adorned with remarkable attention to detail, Latin inscribed text, alchemical symbology, a Prague inspired Magisterium etched underneath the hood, mechanical gear driven dials, colorful pictographs, with Roman and Arabic numbers The casing is covered with ornate details and engravings from edge to edge.virtually hundreds of intricate details The device measures approx 3.5" in diameter and is approx 1.75" thick. The Subtle Knife and 3 The Amber Spyglass In 2007 New Line Cinema released the movie based on his first novel.The GOLDEN COMPASS starring Nicole Kidman, Daniel Craig and Dakota Blue Richards.as sci-fi, fantasy, adventure movie In the movie.the character Lyra, played by Dakota Blue Richards, uses a device.an Alethiometer, a "truth measurement" is the main prop in the movie and an integral part of the plotThis is a high quality replica of the Alethiometer used in the movie The Golden Compass Noble Collections.a company that specializes in the creation of limited edition movie replicas, props and other movie collectibles made this as a limited edition piece.no longer in production and extremely hard to come by in the secondary collectors market These are rarely seen up for sale and t his is not some cheaply made replica. ![]() Northern Lights (published as The Golden Compass in North America) 2. Phillip Pullman wrote an epic trilogy of Fantasy Novels known as the Dark Materials consisted of 3 novels, 1. ![]() Rare and Hard to Find Limited Edition Movie Prop Replica GOLDEN COMPASS ALETHIOMETERHere is a little History on this device. ![]() ![]() ![]() ![]() When I've been invited in, I've even gotten to bang some of their mothers on the way out. After I've kissed the poor girl until her shoes are about to explode off her feet, I ask if they want me to come inside with them or if they'd like to fuck in the backseat? Having your face on TV playing for a popular team is a huge aphrodisiac to most young women, so when I put my hand on their inner thigh and ask if they'd like a kiss, they all say yes. I always offer to drive them home so they get there safe, ensure they're at least 18 years old, and then seduce them. My wife always hires them, so they don't know they'll be babysitting for a footy star. My latest adventure has been to screw every one of our babysitters. If I can get her to fuck another guy, she can't get pissed at me when I fuck other women, right? ![]() I hinted that maybe she could go with him when we were out together, but she just laughed it off as a joke. One of my teammates mentioned that he thought she was hot as fuck and wouldn't mind a go at her. I've tried to get her to loosen up and expand her sexual horizons, but she won't. My wife's a bit of a straight-laced Christian girl. Plus, I love my wife sucking the juices of some slut I've just fucked off my wang. It's the naughtiness of the forbidden fruit that I like so much. I love my wife and kids dearly, but stray pussy is always so delicious. It's the whole 'have your cake and eat it' thing. It gets a little hard to resist, and I don't really want to. As a semi-famous footballer, there's always a bevy of women willing to drop their knickers and jump into bed with you. Our marriage is pretty good, except I'm a bit of a slut, you know? That was five years ago, we've had another kid since then, and she's pregnant with our third. ![]() We'd been high school sweethearts, so when she said she was preggers, I asked her to marry me. I knocked my now wife up celebrating one of those finals wins. I even got to play a couple of finals, so life is good. They were paying me an awful lot of money to play reserve grade and to fill in with the firsts as needed. I just couldn't seem to nail down that permanent spot, though. Wing man is out? John can cover that, too. Your gun forward gets suspended, and John will do a perfectly adequate cover job. Your best defender goes down for a couple of weeks? John's your man to plug the gap. The proverbial fringe player, the Mr Fixit, of the team. The problem is, I'm a nearly guy, you know? Never quite good enough or consistent enough to hold down a full-time spot in the team. I'm a talented football player recruited by one of Brisbane's two major football teams. If this offends you, this is not a story you need to read. The characters, who are mainly a mixture of gay, gay curious, bisexual, or bisexual curious, trans or trans friendly, call each other names such as fag, fagboy, poofter, pooftah, queer, and worse. Warning: There is a lot of use of words that are currently deemed derogatory, insulting, and demeaning. Any resemblance to actual people alive or dead is merely coincidence. To Disney we say, to quote the classic line in the movie, “ Don’t FUCK with the babysitter.It should go without saying but: This is a work of fiction. Blog it, tweet it, scream it to the mountaintops. Pass on the message to Disney that this is most certainly NOT cool. This madness must be stopped! So I am starting a movement. I really can’t imagine any circumstances where this would be ok, but if there were they would most certainly not be with miss Miley at the helm. Now that you’re up to speed on it’s awesomeness. If you have not seen this movie please rush out and watch it. Not to mention a list of other badass names. It stars kick-ass 80s movie & TV staples, Elisabeth Shue, Keith Coogan, Stacy Keanan, and a pre-Rent Anothony Rapp. The awesome story of a Babysitter and her adventures with her three wards in Chicago. Most followed one 80s teen movie formula or another (mixed social classes chill, friends hang out & overcome adversity of some sort, or poor kid falls for rich kid and serious lessons in love are learned).īut one 80s teen movie was in a category of it’s own. Now the 80s definitely saw its share of AWESOME movies. I thought that last summer’s nonsense about the 21 Jump Street movie had died down, but now this, this is just BLASPHEMOUS. ![]() ![]() ![]() In addition, applying a dilution series of a purified protein of known concentrations can be used to allow a more precise estimate of protein concentration. A semi-quantitative estimation of a protein can be derived from the size and colour intensity of a protein band on the blot membrane. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. At least 8–9% of all protein-related publications are estimated to apply western blots. The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein-modifications (such as post-translational modifications). Applications Western blot HIV test where first two strips are negative and positive controls followed by actual tests Between 19 "it has been mentioned in the titles, abstracts, and keywords of more than 400,000 PubMed-listed publications" and may still be the most used protein-analytical technique. The Towbin group also used secondary antibodies for detection, thus resembling the actual method that is almost universally used today. Neal Burnette in 1981, although the method itself was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland. Similarly, detection of RNA is termed as northern blot. The name western blot is a play on the Southern blot, a technique for DNA detection named after its inventor, English biologist Edwin Southern. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA). The secondary antibody is visualized through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein. A secondary antibody is added which recognizes and binds to the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a complicated protein combination. ![]() The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Chameleon Kit: Mix and match Chameleon 700 and Chameleon 800 to create the best protein ladder for your experimental needs.Analytical technique used in molecular biology Western blot workflow.Chameleon 800: 6 multi-colored, pre-stained proteins.Chameleon 700: 7 multi-colored, pre-stained proteins.Chameleon Duo: 11 multi-colored, pre-stained bands.Protein Ladders Provide Reliable MW Marker Sequences Not sure which protein marker to choose? Visit How to Choose the Right Protein Ladder. For special cases, use the Chameleon Kit to customize a two-color protein ladder that suits the needs of your experiment. Chameleon Duo can be used to perform two-color visible and near-infrared detection. Easily visualize gel migration and protein sizeĬhameleon Pre-stained Protein Ladders are optimized for use with Bis-Tris and Tris-Glycine gels.Ĭhameleon 700 or Chameleon 800 protein ladders can be used for one-channel analysis. ![]() Ensure good size coverage with Chameleon ladders ranging from 8 kDa to 260 kDa.Estimate molecular weights of your proteins using these broad-range recombinant proteins.Chameleon near-infrared (NIR) pre-stained protein ladders offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared fluorescent detection. ![]() ![]()
![]() ![]() Our cars are like usthey need proper, routine checks to stay healthy. Here’s a basic seasonal guide to auto maintenance that you can DIY at home or review with your local mechanic. Tire's condition and tread depth: Periodically.īrake Fluid: every 2 years, or 24,000 miles.īrake pads/brake shoes: When the brake pads/brake shoes' thickness is at the minimum safe thickness. Engine inspection for damages, leaks, and maintenance checks must take place too. ![]() Most vehicles manufacturer recommend replacing the coolants every 2 years, or 24,000 miles. The inspector must check the belt operations. The inspector must also check the hoses for any leaks or other issues. The inspector must check the exhaust manifold, tailpipes, muffler, and catalytic converter for damage, leaks or cracks. Monthly Vehicle Maintenance Checklist Check your tire pressure and condition. Most vehicles manufacturer recommend replacing the vehicle's oil and oil filter every 3000 miles. ![]() Most vehicles manufacturer recommend replacing the vehicle's transmission fluid every 2 years, or 24,000 miles.Ĭheck the oil level and change the oil if necessary. Most vehicles manufacturer recommend checking the vehicle's power-steering fluid every 3 months. Most vehicles manufacturer recommend checking the vehicle's battery every 4-5 years. Periodically inspect your vehicle's lights to make sure that there is no burnt bulb and to make sure that all lights are all working properly. Routinely checking and changing your cars motor oil is essential to keeping an engine in good, running condition. It is important to make sure that your windshield wipers are working properly, so make sure to replace them as soon as they are worn out. Check your oil level each month and change it. Most vehicle's manufacturer recommend replacing the timing belt between 60,000 and 90,000 miles. Most vehicle's manufacturer recommend replacing the spark plugs between 30,000 and 100,000 miles. Most vehicle's manufacturer recommend replacing the vehicle's air filter every year, or every 24,000 miles. ![]() ![]() ![]() ![]() 940.302 (2), if the court finds that the crime was sexually motivated, as defined in s. 971.23 or objections to the admissibility of statements of a defendant shall not be made at a preliminary examination and not until an information has been filed.ĩ71.31(5)(c)(c) In felony actions, objections based on the insufficiency of the complaint shall be made prior to the preliminary examination or waiver thereof or be deemed waived.ĩ71.31(6)(6) If the court grants a motion to dismiss based upon a defect in the indictment, information or complaint, or in the institution of the proceedings, it may order that the defendant be held in custody or that the defendant's bail be continued for not more than 72 hours pending issuance of a new summons or warrant or the filing of a new indictment, information or complaint.ĩ71.31(7)(7) If the motion to dismiss is based upon a misnomer, the court shall forthwith amend the indictment, information or complaint in that respect, and require the defendant to plead thereto.ĩ71.31(8)(8) No complaint, indictment, information, process, return or other proceeding shall be dismissed or reversed for any error or mistake where the case and the identity of the defendant may be readily understood by the court and the court may order an amendment curing such defects.ĩ71.31(9)(9) A motion required to be served on a defendant may be served upon the defendant's attorney of record.ĩ71.31(10)(10) An order denying a motion to suppress evidence or a motion challenging the admissibility of a statement of a defendant may be reviewed upon appeal from a final judgment or order notwithstanding the fact that the judgment or order was entered upon a plea of guilty or no contest to the information or criminal complaint.ĩ71.31(11)(11) In actions under s. All issues of fact arising out of such motion shall be tried by the court without a jury.ĩ71.31(5)(a)(a) Motions before trial shall be served and filed within 10 days after the initial appearance of the defendant in a misdemeanor action or 10 days after arraignment in a felony action unless the court otherwise permits.ĩ71.31(5)(b)(b) In felony actions, motions to suppress evidence or motions under s. (3), a motion shall be determined before trial of the general issue unless the court orders that it be deferred for determination at the trial. The motion to suppress evidence shall be so entertained with waiver of jeopardy when it appears that the defendant is surprised by the state's possession of such evidence.ĩ71.31(3)(3) The admissibility of any statement of the defendant shall be determined at the trial by the court in an evidentiary hearing out of the presence of the jury, unless the defendant, by motion, challenges the admissibility of such statement before trial.ĩ71.31(4)(4) Except as provided in sub. The court may, however, entertain such motion at the trial, in which case the defendant waives any jeopardy that may have attached. ![]() (5), defenses and objections based on defects in the institution of the proceedings, insufficiency of the complaint, information or indictment, invalidity in whole or in part of the statute on which the prosecution is founded, or the use of illegal means to secure evidence shall be raised before trial by motion or be deemed waived. Criminal procedure-proceedings before and at trial.ĩ71.31(1)(1) Any motion which is capable of determination without the trial of the general issue may be made before trial.ĩ71.31(2)(2) Except as provided in sub. View our newest version here 2011 Wisconsin CodeĬhapter 971. ![]() |
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